Beta-Actin mouse mAb #50201

beta-actin Antibody

Background: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Beta-actin is one of the two nonmuscle cytoskeletal actins and is relatively stable and appears to remain at normal levels regardless of experimental treatment. It is generally used as an internal control for experiments.

Formulation: 100uL of mouse monoclonal IgG in 1mg/mL of 10mM Tris-HCl, pH 8.0, 150mM NaCl, 25% glycerol, 0.02% sodium azide.

Storage: Store the product at -20°C. Product is stable for about 6 weeks at 2-8°C as an undiluted liquid. Avoid repeated freezing and thawing.

Note: For maximum recovery of product, centrifuge the original vial prior to removing the cap.

ELISA: A working concentration of 0.5~2μg/ml is suggested. Detection sensitivity 1ng/ml.

Immunoblot Analysis: 0.5~2μg/ml(1:500-1:2000)

Immunoblot Protocol

Perform SDS-polyacrylamide gel electrophoresis (SDS-PAGE) on samples and transfer the proteins to a PVDF membrane. Block the blotted PVDF membrane in freshly prepared PBS containing 5% milk with for 1 hour at room temperature with constant agitation. Incubate the PVDF with the recommended dilution of beta actin antibody(#50201) diluted in freshly prepared 5% milk for 1 hour at room temperature or overnight with agitation at 2-8°C. Wash the PVDF 3 times with PBS. Incubate the PVDF in the secondary reagent of choice (a goat anti-mouse secondary antibody conjugated to HRP, Catalog # K009, 1:3000 dilution was used) in 5% milk for 1 hour with agitation at room temperature. Wash the PVDF 3-5 times with PBS. Incubate the PVDF in ECL substrate for 1 min and cover the membrane by PE film. In the darkroom, expose the PVDF to Autoradiography film for 5s~ 5 min to visualize results.

本产品仅供研究使用/For Research Use Only.